Application

Human IgG, Fab Fragment, Plasma has been used in enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Immunoglobulin G (IgG)initiatesthe classical pathway of the complementsystem and eliminates virus particles and toxins. Itcontrolsantibody-dependent cell-mediated cytotoxicity(ADCC)and might be correlated toallergy.Digestion ofIgGby papaingeneratesfragment antigen-binding (Fab) whereas pepsin digestion ofIgGreleases crystallizable(Fc)fragmentand a single dimeric F(ab)₂.MaternalIgGis transferred tothefetusvia the placentaand is crucial forthe immunedefenseof the neonate againstinfections. IgGlevelsinsevere acute respiratory syndrome coronavirus (SARS-CoV)2 infected patientsmay be useful to understand the severity and prognosis ofcoronavirus disease 2019 (COVID-19).

General description

Immunoglobulin G (IgG)is the most crucialglycoproteinantibody. It has the longest serumhalf-life compared to otherimmunoglobulins. IgG is presentinhuman serum and alsoidentified inthe bloodstream. It containsfour polypeptide chains, wherein the light and heavychains arelinked by disulfidebonds.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

2 mg in Plastic ampoule

Physical form

In 150 mM NaCl, 20 mM sodium phosphate buffer, 0.05% NaN₃, pH 7.4.

Preparation Note

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies of HIV and HCV.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Warning

Toxicity: Standard Handling (A)